Micrococcal Nuclease S7
Catalog No. ABIN5706732
Supplier Product No.: mb-116-0015
Quick Overview for Micrococcal Nuclease S7 (ABIN5706732)
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Species
- Staphylococcus aureus
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Supplier Product No.
- mb-116-0015
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Supplier
- Rockland
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Purpose
- Micrococcal Nuclease S7
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Characteristics
- Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
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Purification
- Purified by chromatography and assayed by SDS-PAGE.
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Unit Definition
- One Unit causes a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.
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Biological Activity Comment
- 6,000 units per mg protein
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Restore with 1 mL deionized water (or equivalent).
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C or at -20° C or colder prior to opening product.
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Expiry Date
- 6 months
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